Prepare Tris Running Buffer

Prepare Tris Running Buffer
Start this procedure 5 min prior to completion of polymerization of the Upper Gel.

1. Add 250 ml of 4x Tris Running Buffer to a 1000 ml graduated cylinder.
2. Add 10 ml. of 10% SDS to the cylinder using a 10 ml pipet.
3. Add RO H2O to 1000 ml.
4. Pour this solution into the bottom reservoir and add magnetic stirrer.
5. Add 150 ml of 4x Tris Running Buffer to a 1000 ml graduated cylinder.
6. Add 6 ml. of 10% SDS to the cylinder using a 10 ml pipet.
7. Add RO H2O to 600 ml. Set aside.