After the Lower Gel has polymerized

After the Lower Gel has polymerized for 60 min:

1. Invert casting stand over sink and rinse out isobutanol with RO H2O.

2. Add ______ ul TEMED and swirl gently to avoid introducing air.
3. Immediately transfer the solution in between the glass plates.
4. Insert combs between the glass plates.
Insert at an angle to prevent air bubbles from becoming trapped at the bottom of teeth.
5. Add as much remaining solution as possible to the top of the gel.
6. Allow gel to completely polymerize for 60 min.