Load Samples

Load Samples
1. Move gel casting stand to sink
2. Pull the combs out- straight upwards.
3. Wash-out the wells three times with the 1x Upper Tris Buffer squeeze bottle.
Fill the wells with this solution.
4. Add rubber gaskets to the Top Reservoir
Notice ridges that sit in the recess.
5. Lower the Top Reservoir onto glass plates in casting stand.
6. Remove cam levers from lower slots and insert into upper slots and turn to tighten.
Be sure cam levers are pushed all the way into slot before tightening.
7. Add 300 ml. Tris Running Buffer to Top Reservoir.
8. Tape the clear plastic sheet with well numbers in the corresponding position
9. Load samples according to the Gel Run Log using a 10 ul Hamilton syringe.
Wash the syringe between samples with 1x Tris Running Buffer.
To assist visualizing the wells and sample load, you can add a wisp of Tracking Dye using an automatic pipeter. Avoid adding too much tracking dye.
1. Gel A:
1. Load samples to Lanes 1-10
2. Load IgG standards to Lanes 11-15 in the the following order: 10, 8, 6, 4 and 2 uL.
3. Load samples to Lanes 16-28.
2. Gel B:
1. Load samples to Lanes 1-10
2. Load 1 ug/ul IgG standards to Lanes 11-15 in the the following order: 2, 4, 6, 8 and 10 uL.
3. Load samples to Lanes 16-28.
3. Gel C:
1. Load samples to Lanes 1-9
2. Load 1 ug/ul IgG standards to Lanes 10-14 in the the following order: 10, 8, 6, 4 and 2 uL.
3. Load samples to Lanes 15-28.
4. Gel D:
1. Load samples to Lanes 1-9
2. Load 1 ug/ul IgG standards to Lanes 10 -14 in the the following order: 2, 4, 6, 8 and 10 uL.
3. Load samples to Lanes 15-28.
10. Be sure to add comments for missed lanes or botched loadings in the Gel Run Log.