Requirement Of SDS PAGE ELECTROPHORESIS

Requirement Of SDS PAGE ELECTROPHORESIS
Reagent: 5% 7.5% 10% 12.5%

RO H2O (ml)	22	14.6	12	9.6
30%/0.8% Acrylamide/Bis (ml)	5	7.5	10	12.5
4x Lower Tris Buffer (ml)	7.5	7.5	7.5	7.5
10% SDS (ul)	300	300	300	300
APS (ul)	150	150	150	150

Materials:
DC Power supply
Precast ready gels
3.TGS electrophoresis buffer(24.8mM Tris Base , 192mMGlycine , 0.1% SDS)
4.Gel stain (Coomassie Blue)
5.Gel Destain Solution(50% H2O, 10% Acetic Acid, 40% Menthol)
6.Pippetters
7.Gel Loading Tips
8.Tubes
9.Razorblade
10.Tupper Wear Container
11.Square Petri Dish
12.Glass Ware to make buffer Solutions
13.Spatulla
14.Sample
15.5x running dye